DNA

Part:BBa_K1975009:Design

Designed by: Joachim Steen Larsen   Group: iGEM16_UNIK_Copenhagen   (2016-10-13)


Initiator protein repA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 148


Design Notes

The sequence was obtained by PCR from the shuttle vector pHT254.


Source

This gene is obtained by the use of PCR from the vector pHT254. This vector is a shuttle vector for both Escherichia coli and B. subtilis.

References